Effect of lipopolysaccharide on viability and secretion function of human umbilical vein endothelial cells
- VernacularTitle:脂多糖对体外培养人脐静脉内皮细胞分泌功能及活力的影响
- Author:
Shanshan LI
;
Peng LI
;
Qifu HUANG
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharides;
Endothelium;
Cells;
Endothelins;
Nitric oxide
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the direct effect of lipopolysaccharide (LPS) on secretion of endothelin-1(ET-1) and nitric oxide by human umbilical vein endothelial cell and cell viability of the secretor. METHODS: The third passage of human umbilical vein endothelial cells were incubated with different concentrations of LPS(1 g/L, 100 mg/L, 10 mg/L, 1 mg/L, 100 ?g/L, 10 ?g/L, 1 ?g/L) for 6 hours, and the culture supernatants were collected. The concentrations of ET-1 were determined by radioimmunoassay, the concentrations of nitric oxide were determined using Greiss's method. The viabilities of cells were measured by MTT method. RESULTS: The concentration of ET-1 (pg/L) of normal control group was 251 64?10 90. The concentrations of ET-1(pg/L) of LPS treated groups were 220 85?19 14, 278 67?15 45, 306 40?11 60, 312 87?33 50, 324 38?17 02, 291 49?14 30, 282 11?13 38, respectively. (each group compared with normal control group, P
