Determination of Valsartan Concentration in Human Plasma With HPLC
- VernacularTitle:高效液相色谱法测定人血浆中缬沙坦的药物浓度
- Author:
Hongmei LIU
;
Yan LIU
;
Rui JIN
;
Zhen WANG
;
Kaoxiang SUN
;
Yicheng LU
- Publication Type:Journal Article
- Keywords:
valsartan;
plasma concentration of drug;
HPLC
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the valsartan concentration in human plasma METHODS:The plasma sample was extracted with a liquid-solid method and determined with HPLC,stationary phase was Hypensil ODS C18(4 6nm?200nm,5?m),mobile phase consisted of acetonitrile and 0 01mol/L KH2PO4 buffer(pH 2 8)(50∶50) The flow rate was 1 5ml/min Detection was performed with fluorescence detector at ?ex 265nm,?em 378nm RESULTS:The retention time of valsartan was about 5 4 minutes,and the linear range of quantity was 0 05～5?g/ml The recoveries of methodology were more than 90%(n=5) Inter-day and intra-day RSD were less then 10%(n=5) CONCLUSION:This method is rapid and accurate It can be applied to determining the plasma valsartan concentration and studying on pharmacokinetics
