High Insulin-induced Insulin Resistance Dependent on the Increasing Glucose Concentration Surrounding the Cells
- VernacularTitle:高胰岛素血症诱导胰岛素抵抗与血糖浓度增加有关
- Author:
Huixia LIU
- Publication Type:Journal Article
- Keywords:
Insulin,receptor;
Insulin resistance;
Blood,glucose
- From:
Journal of Chinese Physician
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of high insulin and high glucose concentrations on glucose transport activity,the expression of insulin signaling peptides and glucose transporter 4(GLUT4) translocation in primary cultured rat adipocytes.Methods Isolated rat adipocytes were cultured for 24h at insulin(10 4?U/ml) and different concentration glucose(5,10,15 and 25mmol).Then the glucose uptake,cellular contents of insulin receptor substrate(IRS) 1/2,phosphatidylinositol 3-kinase 85 subunit(p85),protein kinase B(PKB) and GLUT4 were measured by Western blotting method.Results These adipocytes treated with insulin and different high concentration glucose had shown to impair glucose uptake in a dose-dependent manner,inhibited cellular IRS1 expression,impaired IRS2 protein expression independent on glucose concentration in the medium,it did not influence the contents of p85,PKB and GLUT4,but decreased GLUT4 translocation.Conclusions Chronic high insulin induced insulin resistance may be caused by the increasing glucose concentration surrounding the cells.The mechanism may be involved in affecting IRSs protein expression and GLUT4 translocation.
