The Clinical Preliminary Application and Analysis for Drug-Sensitive Test and Detecting Drug Resistance Genes to Mycobacterium Tuberculosis
- VernacularTitle:结核分支杆菌药敏和耐药基因检测的临床应用和分析
- Author:
Wei WANG
;
Hongmin LI
;
Xueqiong WU
- Publication Type:Journal Article
- Keywords:
Tuberculosis;
Mycobacterium tuberculosis;
Drug resistance;
Gene expression;
PCR-SSCP
- From:
Journal of Chinese Physician
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the clinic application value of detection of rPOB,katG and rPSL resistance genes to M tuberculosis by polymerase china reaction-single stranded conformation polymorphism (PCR-SSCP).Method The drug-resistance tests of M tuberculosis clinical isolates from 141 patients with the tuberculosis was analyzed by Bactec-960 rapid culture,drug-sensitive and PCR-SSCP techniques.Results As nearly As one of three patients with priliminary treatment had resistance to one kind of the drugs tested at least.The mutation rates of RFP,INH and SM drug-sensitive isolates were 6 5%,11 4% and 27 2% respectively.That of the drug-resistant isolates were 60 2%,45 7% and 62 7%.As drug-resistant concentration heightened,so mutation rate of genes increased.Conclusions Detecting the resistance genes was a new explore in guiding treatment,and multiple drug-sensitive test combinative use have cooperativity each other.It might become good test for clinical management.
