Effects of oxidized HDL on the levels of MCP-1, ICAM-1 and free calcium in cultured human umbilical venous endothelial cells
- VernacularTitle:氧化HDL对内皮细胞MCP-1、ICAM-1含量和细胞内游离钙浓度的影响
- Author:
Houyuan HU
;
Yunzhe CHEN
- Publication Type:Journal Article
- Keywords:
Lipoproteins;
Endothelium, vascular;
Calcium;
Monocytes;
Cell adhesion molecules
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of oxidized high-density lipoprotein (oxHDL) on the expression of monocyte chemoattractant protein-1(MCP-1) and intercellular adhesion molecule-1(ICAM-1) and intracellular free calcium concentration ([Ca 2+ ]i) level in cultured human umbilical venous endothelial cells(HUVECs). METHODS: The MCP-1 protein content in the medium of conditioned HUVEC was measured by ELISA, and the ICAM-1 on HUVECs was detected by indirect immunofluorescence, and [Ca 2+ ]i was determined by Fluo-3/AM, the injury of cells was observed by scanning electron microscopy (SEM).RESULTS: oxHDL could induce the expression of MCP-1 and ICAM-1 in HUVECs. In oxHDL group (HUVECs were incubated with 100 mg protein/L oxHDL for 24 h), the levels of MCP-1, ICAM-1 and [Ca 2+ ]i increased by 160%, 60% and 70% respectively compared with the control group ( P
