Effect of continuous CO_2 pneumoperitoneum on tumor cell port site implantation in laparoscopic surgery in a murine model
- VernacularTitle:持续性CO_2气腹影响胃癌细胞在腹腔镜切口种植的实验研究
- Author:
Minhua ZHENG
;
Hao WANG
;
Hongwei LI
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Neoplasm seeding;
Surgical procedures, laparoscopic;
Pneumoperitoneum, artificial
- From:
Chinese Journal of General Surgery
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine whether the pressure of continuous CO 2 pheumoperitoneum (CCP) affects port site implantation of tumor cells.Methods Male SD rats were injected intraperitoneally with gastric cancer cells (cell line SGC 7901). Continuous CO 2 pneumoperitoneum at 15*!mm*!Hg or 30*!mm*!Hg for 5*!mins,60*!mins,120*!mins and 180*!mins with different number of tumor cell injection(10 4/ml, 10 6/ml respectively) was then established. Several samples of peritoneal washing served as positive control.All collecting dishes were incubated at 37℃ for one week.Results Dishes were tumor cell positive on which the tumor cells were collected from rats undergoing continuous flows of CO 2 gas (5*!L/min) at pneumoperitoneum of 30*!mm*!Hg for 60 mins or longer and all peritoneal washing samples showed tumor growth also. Conclusion It suggests that the number of tumor cells, pneumoperitoneum pressure and the duration may affect the port site implantation.
