Investigation of the effect of VEGF antisense oligodeoxyribonucleotides in ischemic retinopathy
- VernacularTitle:血管内皮细胞生长因子反义寡聚脱氧核苷酸在缺血性视网膜病变中的作用
- Author:
Ti WANG
;
Lirong HAN
- Publication Type:Journal Article
- Keywords:
Retinal diseases;
Endothelial growth factors;
Oligodeoxyribonucleotides,antisense;
Retinal neovascularization;
Disease models,animal
- From:
Chinese Journal of Ocular Fundus Diseases
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of suppression of ischemia induced retinal neovascularization by VEGF antisense oligodeoxyribonucleotides. [WT5”HZ]Methods [WT5”BZ]Mouse models of hyperoxia induced ischemic retinopathy were established. Retrobulbar injections were performed with VEGF antisense oligodeoxyribonucleotides or NS in 4 groups:normal control and various doses respectively. The nuclei of new vessel buds extending from the retina into the vitreous in different groups were counted and compared under the light microscope. Results There were plenty of new vessel buds in the eyes of mice in hyperoxic condition., while the number of the nuclei of new vessel buds is less in the murine eyes with retrobulbar injection of VEGF antisense oligodeoxyribonucleotides,especially the nuclei were redused with 59.3% in eyes with large dose. Conclusion The proliferation of retinal new vessel may be suppressed by using the retrobulbar injection of VEGF antisense oligodeoxyribonucleotides.
