The culture and identification of astrocytes of human optic nerve in vitro
- VernacularTitle:人眼视神经星形胶质细胞的体外培养及鉴定
- Author:
Jianliang ZHENG
;
Jie ZHANG
;
Yan GUO
;
Al AT
- Publication Type:Journal Article
- Keywords:
Optic nerve;
Astrocytes;
Cell culture
- From:
Chinese Journal of Ocular Fundus Diseases
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To culture astrocytes of human optic nerve and establish the cell lines for further study of healing process after optic nerve trauma. [WT5”HZ]Methods [WT5”BZ]Astrocytes of infantile optic nerve were cultured by tissue inoculation or tissue digestion with 0.25% trypsin and 0.06% EDTA. The second and fourth passage cells were stained with HE and anti GFAP, S 100 protein, vimentin, and CD34 antibodies. [WT5”HZ]Results The trypsinized astrocytes of infantile optic nerve reached confluence in 7 days. The cultured cells were in polygonal shape with processes and the cytoplasm was abundant. These cells were positive in GFAP, S 100 protein and vimentin staining, and negative in CD34 staining. Conclusions Astrocytes of human optic nerve can be successfully cultured by trypsinization rather than tissue inoculation.
