Role of nuclear factor ?B on the expression of interleukin-6 in mouse mesangial cells induced by interleukin-1?
- VernacularTitle:NF-?B活化在IL-1?诱导的小鼠肾小球系膜细胞表达IL-6中的作用
- Author:
Hanshi XU
;
Rengao YE
;
Qiongqiong YANG
;
Lin SUN
;
Niansheng YANG
;
Youji LI
;
Lixia ZENG
- Publication Type:Journal Article
- Keywords:
Interleukin-1;
Interleukin-6;
NF-Kappa B;
Kidney glomerulus;
Mice
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the regulatory role of nuclear factor ?B (NF-?B) in the expression of interleukin-6 in mesangial cells (MC) induced by interleukin-1?.METHODS: Activation of NF-?B was measured by electrophoresis mobility shift assay (EMSA). RT/PCR and ELISA were used to detect IL-6 mRNA expression and IL-6 production, respectively.RESULTS: rhIL-1? could rapidly stimulate the activation of NF-?B in MC, and increase the expression of IL-6 mRNA and protein. PDTC, one of the inhibitor of NF-?B, could inhibit the expression of IL-6 in mRNA and protein in MC stimulated by rhIL-1?.CONCLUSION: IL-6 expression induced by IL-1? may be regulated by NF-?B in MC, NF-?B may modulate the immune-inflammatory reaction in glomerular disease.
