Effects of 6,7-dimethoxycoumarins on Cell Proliferation and Cell Generation Cycle of Human Lung Carcinoma Cell in Vitro
- VernacularTitle:茵陈素对肺癌细胞增殖和细胞周期的影响
- Author:
Yunzhong TAN
;
Guohua DAI
;
Jun LI
- Publication Type:Journal Article
- Keywords:
dimethoxycoumarins;
lung carcinoma;
inhibition action;
cell cycle
- From:
China Pharmacy
1991;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of 6,7-dimethoxycoumarins on the proliferation and the cell cycle of human lung carcinoma cell(PA)in vitro METHODS:The effects of 6,7-dimethoxycoumarins on the cytomorphology,growth and cell cycle of PA cells were evaluated by MTT assay and flow cytometry in vitro RESULTS:6,7-dimethoxycoumarins could inhibit the proliferation of PA cells in a dose-dependent manner The inhibition action of 6,7-dimethoxycoumarins plateaued at 160?g/ml(52 4% inhibition) The agent in dose of 80?g/ml led to a decline in the proportion of cells to those in G2/M phase,made the cells remain in G0/G1 phase and reduced the proliferation index(PI) of PA cells CONCLUSION:In vitro,6,7-dimethoxycoumarins can inhibit the proliferation of PA cells,which leads to arrest of the cells in G0/G1 phase by way of inhibiting DNA synthesis
