Directly Injury Effects of Lipopolysaccharide on Human Endothelial Cells in Vitro
- VernacularTitle:脂多糖对人脐静脉血管内皮细胞的直接损伤作用
- Author:
Lei YAO
;
Yu SUN
;
Shan CHENG
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharide;
Vascular endothelial cells;
Injury;
Umbilicus vein
- From:
Journal of Chinese Physician
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the directly injury effects of lipopolysaccharide(LPS) on human vascular endothelial cells(HVEC).Methods Lipopolysaccharide with different concentrations added into cultured vascular endothelial cells of human umbilical vein,at different phase after lipopolysaccharide being added,the concentrations of nitric oxide(NO),lactic acid dehydrogenase(LDH) were measured,and the adhesion of HVEC to polymorphonuclear leucocytes(PMN) were observed,the morphological changes of HVEC were detected by phase-contrast microscope.Results The NO content,LDH activity were obviously elevated with increase of LPS concentration(P
