Alteration of cardiac sarcoplasmic reticulum function in vitamin D_3-induced calcium overload rats
- VernacularTitle:维生素D_3诱导的钙超载大鼠心肌肌浆网功能变化
- Author:
Yongfen QI
;
Linwang DONG
;
Lin XUE
;
Xiaohong WANG
;
Minggui FU
;
Chaoshu TANG
- Publication Type:Journal Article
- Keywords:
Calcium;
Myocardium;
Sarcoplasmic reticulum;
Vitamin D
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study alterations of cardiac sarcoplasmic reticulum (SR) function in vitamin D 3-induced calcium overload rats. METHODS: The Ca-overload rat models were prepared by vitamin D 3 plus nicotine. Cardiac SR was seperated by centrifuging. The measurement of SR Ca 2+ uptake and Ca 2+ release activities were preformed by the Millimore filtration technique. Specific SR [ 3H]-ryanodine binding capacity was measured by radioligand method. RESULTS: Compared with control,myocardial calcium content in calcium overload rats increased by 78%( P
