Effect of Jiunaoning Injection on apoptosis and changes in cytosolic calcium induced by hypoxia / hypoglycemia in SH-SY5Y human neuroblastoma cells
- VernacularTitle:救脑宁注射液对神经细胞凋亡及胞浆钙变化的影响
- Author:
Liyun HE
;
Jiping FAN
;
Sulun SUN
;
Qifu HUANG
;
Shuoren WANG
;
Lingqun ZHU
;
Zhuang ZHANG
;
Wei CUI
;
Ying GAO
;
Yihua ZOU
- Publication Type:Journal Article
- Keywords:
Drugs, Chinese herbal;
Cells;
Apoptosis;
Calcium
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of Jiunaoning Injection on apoptosis and the changes in cytosolic calcium induced by hypoxia/hypoglycemia in SH-SY5Y human neuroblastoma cells. METHODS: The apoptosis and the changes in cytosolic calcium were induced by hypoxia and hypoglycemia .Apoptosis was measured via flow cytometry(FCM), and cytosolic calcium was determined by Fluo-3 fluorometry. RESULTS: The hypoxia/hypoglycemia induced apoptosis and increased the cytosolic calcium in SH-SY5Y cells. Jiunaoning Injection inhibited the apoptosis of the SH-SY5Y cells and decreased the concentration of cytosolic free calcium. CONCLUSION: Hypoxia/hypoglycemia induced apoptosis and increase in cytosolic free calcium in SH-SY5Y cells, which were significantly inhibited by Jiunaoning Injection.
