Changes in endogenous nitric oxide and effects of inhaled nitric oxide on pulmonary artery hypertension in chronically hypoxic rats
- VernacularTitle:慢性低氧性肺动脉高压大鼠内源性一氧化氮代谢及其外源性吸入的研究
- Author:
Shengxiang GUO
;
Xinzh WENG
- Publication Type:Journal Article
- Keywords:
Pulmonary artery;
Hypertension, pulmonary;
Anxia;
Nitric oxide;
Endothelins
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of nitric oxide (NO)in the development of chronically hypoxic pulmonary artery hypertension (PAH) and the hemodynamic effects of inhaled NO on pulmonary circulation. METHODS: 67 male adult SD rats were randomly divided into 7 groups: (1) control ( n= 9);(2) chronically intermitent hypoxia (CIH, 6 h/d, 7 d/w) 1 week( n= 7); (3) CIH 2 weeks ( n= 11); (4) CIH 3 weeks ( n= 11); (5) CIH 1 week+L-NAME (NO synthase inhibitor, 30 mg/kg, by gavage, n= 10); (6)CIH 3 weeks+L-Arg (NO precursor, 10 mg/kg, by gavage, n= 9); (7) CIH 3 weeks+inhaled NO (0.0004% for 20 min, n= 10) to determine the mean pulmonary artery pressure (MPAP), weigh the right ventricle (R) and ventricular segment plus left ventricle (S+L), and calculate R/(S+L) (g/g) and R/Wt (Wt: body weight, g/kg). RESULTS: 1.MPAP increased compared with control [(3 2?0.3 ) kPa vs (1.8?0.3) kPa, P
