Inhibitory effect of ATP on proliferative signaling in immortalized human fibroblasts
- VernacularTitle:ATP抑制不死化人成纤维细胞增殖信号转导机制的研究
- Author:
Junwu LI
- Publication Type:Journal Article
- Keywords:
Adenosine triphosphate;
Cells;
Calcium channels;
Potassium channles;
Inositol 1,4,5-trisphosphate
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the inhibitory effects of ATP on proliferation signaling in immortalized human fibroblasts. METHODS: Immortalized human fibroblasts were treated with ATP, ATP conbined with calcium or potassium channel antagonists, respectively. The intracelluar-free calcium ([Ca 2+ ]i), inositol 1,4,5-trisphosphate(IP 3) levels and cell viability were detected at different time points. RESULTS: ATP significantly increased the [Ca 2+ ]i and decreased the IP 3 level in immortalized human fibroblasts, especially at initial stage ( P
