Study on biological activity of thrombopoietinⅡ in vivo
- VernacularTitle:血小板生成素Ⅱ体内生物活性研究
- Author:
Liye ZHONG
;
Yunxian CHEN
;
Xueyun ZHONG
;
Tianhao LIU
;
Ruiming OU
;
Hongyun ZHAO
;
Weiwei SU
;
Wei LIN
;
Xin DU
;
Peili XU
- Publication Type:Journal Article
- Keywords:
Thrombopoietin;
Blood platelets;
Mice
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the biological activity of thrombopoietin Ⅱ(TPOⅡ) in vivo , which consists of two new kinds of ligand binding with thrombopoietin receptor. METHODS: Purified ligandⅠof TPOⅡ, artificial compound ligandⅡ of TPOⅡand rhTPO were injected into purebred Babl/c mice respectively in 7 days by intraperitoneal injection once for a day. Then the biological activity of TPOⅡ was analyzed by measuring peripheral platelet counts by the end of the seventh day. RESULTS: On the seventh day, the platelet counts of mice treated by ligandⅠof TPOⅡ were higher than that in the negative control group( P 0.05). On the fourteenth day, the platelet counts increased in two all experimental groups of TPOⅡcompared with negative control group( P 0.05). Moreover the platelet counts of mice in two experimental groups of TPOⅡ and the positive group showed increase with experimental days. CONCLUSION: The purified ligandⅠof TPOⅡ had obvious activity in increasing platelet production, which is not different from the effect of rhTPO.
