The Relationship Between Anti inflammatory Effects of Antipsoriatic Drugs and 5－Lipoxygenase Products
- VernacularTitle:环孢素A、雷公藤内酯醇及红霉素与5-脂氧合酶产物的关系
- Author:
Lianwen SUN
;
Jiarun ZHENG
;
Yun CHEN
- Publication Type:Journal Article
- Keywords:
Arachidonic acid;
5－lipoxygenase;
Leukotriene B4;
Hydroxyeicosatet raenoic acids;
Cyclosporin;
Triptolide;
Erythromycin
- From:
Chinese Journal of Dermatology
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To Investigate the effects of antipsoriatic drugs on 5 lipoxygenase(5－LO) and set up a relevant pharmacodynamic method. Methods 5－ LO products, leukotriene B4 (LTB4) and 5 hydroxyeicosatetraenoic(5－HETE), we re determined by RP－HPLC to represent 5－LO activity. Results Cyclosporin A( CyA) and triptolide(T0) inhibited the production of LTB4 and 5－HETE in a dose dependent manner, while erythromycin did without dose dependence. The 50％ inhibitory concentration values(IC50) of CyA inhibiting LTB4 and 5－HETE were 3 8.0? g/mL and 0.96? g/mL, respectively. The IC50 of T0 inhibiting LTB4 and 5－ HETE were 2.3? 10－6? g/mL and 1.14? 10－6? g/mL, respectively. Conclusio ns The anti inflammatory effect of Tripterygium wilfordii Hook.f. may be partl y explained by its inhibition of 5－LO activity. The anti inflammatory effect of CyA has no clinical significance since the inhibitory concentration of CyA h as exceeded its pharmacological limitation. Erythromycin has no effect on 5－LO activity.
