Quantitative Detection of Serum HBV-DNA in Patients with Chronic Hepatic Disease and Its Clinical Application
- VernacularTitle:慢性肝病患者血清HBV-DNA定量检测及临床应用
- Author:
Ting WU
;
Yuli CHEN
;
Ciyang WANG
- Publication Type:Journal Article
- Keywords:
Hepatitis,Viral,Type B;
Quantitative PCR
- From:
Journal of Chinese Physician
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship among the content of serum HBV-DNA and the different degree of chronic liver damage,and the marks of hepatitis B virus and to explore its value in the evaluation of therapy.Method Fluorescence quantitative PCR was used to measure the concentration of sera HBV-DNA in 113 patients infected by HBV.Results The serum HBV-DNA in mild chronic hepatitis group was the highest.In the patients with aggravating liver damage,the serum HBV-DNA concentration was decreased gradually.It was indicated that serum HBV-DNA concentration in the patients with HBeAg positive was significantly higher than that of HBeAg negative.However,there were no evident relationship among HBV-DNA concentration,ALT,AST activities and different Child's classification of cirrhosis.The patients with lower serum HBV-DNA concentration before treatment had a better outcome.The therapeutical effects were correlated with the serum of viral DNA concentrations.Conclusions The quantitative detection of serum HBV-DNA has instructive value in understanding the relationship among the clinical status of chronic hepatic disease,the duplication level of hepatitis virus and evaluating therapy.
