Gentamicin and Its Metabolite Effect [Ca~(2+)]i in Isolated Outer Hair Cells
- VernacularTitle:庆大霉素及其代谢产物对外毛细胞内游离钙浓度的影响
- Author:
Xianming CHEN
;
Jinling WANG
;
Ling QU
- Publication Type:Journal Article
- Keywords:
Gentamicin Outer hair cellsCalcium Confocal microscope
- From:
Journal of Audiology and Speech Pathology
1998;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between [Ca2 +]i and gentamicin ototoxicity. Methods Isolated outer hair cells were loaded with [Ca2 +]i - sensitive fluorescence dye Fluo- 3, and confocal laser microscopy was used to determine [Ca2 +]i when K +- Hanks、gentamicin or its metabolite were added. Results Gentamicin decreased [Ca2 +]i in short time, and inhibited inward calcium current induced by K + . On the contrary, metabolized gentamicin increased [Ca2 + ] i for a long period. It did not effect calcium current induced by K + . Conclusion Metabolized gentamicin increased [Ca2 + ]i, which may result in its ototoxicity.
