Histochemical study on the change in lymphatic motion and nitric oxide synthase in the early phase of acute rat endotoxemia
- VernacularTitle:急性内毒素血症早期大鼠淋巴管运动变化及其一氧化氮合酶组织学研究
- Author:
Guizhi SHI
;
Baoheng WANG
;
Liping ZHANG
;
Guiying YU
;
Zhenpin LI
- Publication Type:Journal Article
- Keywords:
Endotoxins;
Lymphatic system;
Nitric oxide;
Rats
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the changes in lymphatic motion and histochemical stain of nitric oxide synthase(NOS) in the early phase of acute rat endotoxemia and explore their relationship. METHODS: After lipopolysaccharide (LPS) was injected into femoral vein, rats' mesenteric lymphatic were inspected within two hours. Paraffin section was stained by HE, frozen section by NADPH diaphorase histochemistry. RESULTS: In LPS group, the mesenteric lymphatic diameter was greater than that in the control and contractile frequency was decreased, the villi central lacteal and submucosal microlymphatics of small intestine was extremely dilated under microscope. In LPS and control group, NOS was positive in lymphatic endothelia and the nerve fibers in the vessels' wall. CONCLUSION: In the early stage of acute endotoxemia, the lymphatic vessles dilated and contractile activity diminished, NOS may play an important role in these changes.
