Determination of Serum Concentrations of Propofol by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定异丙酚血清浓度
- Author:
Shuomin JIANG
;
Zengshou WANG
;
Shengwei JIN
- Publication Type:Journal Article
- Keywords:
propofol;
serum concentration;
RP-HPLC
- From:
China Pharmacy
1991;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:A RP-HPLC method was established for determination of serum concentration of propofol in man METHODS:The mobile phase consisted of methanol-water(68∶32v/v)The detection was carried out at wave length 258nm and flow rate 1ml/min with carbamazepine as internal standard RESULTS:The retention times of propofol and carbamazepine were 9 20 and 5 16 min,respectively The mean recovery of propofol was 99% The within-day and inter-day variations were all less than 10% Propofol and carbamazepine were seperated well The assay linearity was obtained in the range of 1～16?g/ml in serum(r=0 9 994) CONCLUSION:The method is sensitive,simple and reliable for the determination of propofol concentration
