Expression of nm23 gene and its significance in heterotransplanted model of retinoblastoma in nude mice
- VernacularTitle:视网膜母细胞瘤裸小鼠移植瘤中nm23基因的表达及意义
- Author:
Xiaowei ZHANG
;
Danian CHEN
;
Chengren LUO
- Publication Type:Journal Article
- Keywords:
Retinoblastoma;
Genes, suppressor, tumor;
Disease models,animal;
Gene expression;
nm23 Gene
- From:
Chinese Journal of Ocular Fundus Diseases
1996;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate nucleoside diphosphate kinase (NDPK ) expression of tumor metastasis suppressor gene nm23 in heterotransplanted model of retinoblastoma(RB) in nude mice,and analyse the correlation between the expression of nm23 gene and the formation and progression of heterotransplanted RB. Methods SP immunohistochemical method was used to detect the expression of nm23 gene product NDPK in 20 tumors of heterotransplanted RB model and normal retinal tissue. Results The negative staining of nm23/ NDPK was found in normal retinal tissue , whereas 100% expression rate in RB tumors with positive number of 48.73?2.37. No statistical significance of the expression of nm23/ NDPK was observed between the intraocular growth phase (I～Ⅲ grade) and invasive phase (Ⅳ～Ⅴ grade) in heterotransplantedRB tumors. Conclusion The function of nm23 gene as a tumor metastasis suppressor in heterotransplanted RB tumors was less prominent ,but it may play a role in carcinogenesis and progrssion of RB and may predict poor prognosis.
