Establishment of nasopharyngeal carcinoma (NPC) cell lines stable expressing NPC-derived LMP1
- VernacularTitle:稳定表达鼻咽癌来源潜伏膜蛋白1基因的鼻咽癌细胞系的建立
- Author:
Ke LAN
;
Runliang GAN
;
Caiping REN
;
Zhiwei HE
;
Lu XIE
;
Kaitai YAO
- Publication Type:Journal Article
- Keywords:
Membrane proteins;
Epstein-Barr virus;
Nasopharyngeal neoplasms
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish nasopharyngeal carcinoma(NPC) cell lines stable expressing NPC-derived latent membrane protein 1(LMP1) gene. METHODS: General expression vector and epithelium-specific expression vector of NPC-derived LMP1 gene were constructed by using recombinant techniques, then transfected these vectors into a poor differentiated NPC cell line named CNE-2 ,integration and expression of N-LMP1 in CNE-2 cells were detected by PCR,RT-PCR and Western blot. RESULTS: (1) General expression vector and epithelium-specific expression vector of NPC-derived LMP1 gene were constructed successfully.(2) It showed that N-LMP1 gene expressed in CNE-2 cells correctly. CONCLUSION: The first NPC cell lines which stable express NPC-LMP1 were established. The cell lines obtained will provide important basis for exploring the role of NPC-LMP1 in nasopharynx carcinogenesis.
