Effects of amlodipine on apoptosis of vascular endothelial cells induced by oxyeterols
- VernacularTitle:氨氯地平抑制氧化型胆固醇诱导的血管内皮细胞凋亡
- Author:
Ming CUI
;
Fengrong CHEN
;
Qinghua SONG
;
Yingba ZHU
- Publication Type:Journal Article
- Keywords:
Cholesterol;
Endothelium, vascular;
Cells;
Apoptosis;
Amlodipine
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the apoptosis of endothelial cells (EC) induced by oxysterols and to examine the effect of amlodipine on the apoptosis induced by oxysterols.METHODS: Light microscope, electron microscope, DNA agarose gel electrophoresis and flow cytometry were used to detect the apoptosis of EC.RESULTS: The characteristic morphological features of apoptosis were observed under light and electron microscope; DNA electrophoresis showed"DNA Ladder"; Flow cytometry showed the sub-G1 peak, apoptotic rate is 32.25% and 23.04% in Triol-treated and 25-OH-treated groups, respectively. While treated EC with amlodipine at the same time, the apoptotic rate decreased significantly. CONCLUSION: Both Triol and 25-OH can induce apoptosis of EC, which can be inhibited by amlodipine.
