ELIZA - SPOT technique applicated to assess the immune response in of oral cholera vaccine in volunteers
- Author:
Nguyen Binh Minh
- Publication Type:Journal Article
- Keywords:
cholera vaccin
- MeSH:
Cholera Vaccines;
Lymphocytes;
Technical Report [Publication Type]
- From:Journal of Practical Medicine
2003;450(4):67-68
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
24 volunteers were divided into 3 groups. Group 1 of 10 subjects was given vaccine in addition of bicarbonate damper, group 2 of 9 subjects was given vaccine and water, group 3 of 5 subjects was given bicarbonate damper. Anti LPS antibody value of V. cholerae 01 of IgA class in ELISA spot was higher significantly than serum ELISA in same time point. Lymphocyte producing IgA antibody persisted about 5 months after immunization. IgM antibody excreting cells had activated promptly rather just 7 days after the first time of talking oral vaccine with a mean value 8 times higher than the mean value before taking vaccine. 100% of studied subjects in 2 groups had had the response of IgA antibody excreted through peripheral blood lymphocytes.
