The regulation of polydatin on [Ca~(2+)]i of myocardial cells in the rat
- VernacularTitle:虎杖甙对心肌细胞钙的调节作用
- Author:
Chunhua JIN
;
Kesen ZHAO
;
Jie LIU
;
Xuliang HUANG
- Publication Type:Journal Article
- Keywords:
Rats;
Myocardium;
Calcium
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To elucidate the mechanism of polydatin(PD) in increasing the contractility of myocardial cells by observing the cytosolic free calcium concentration ([Ca 2+ ]i) of myocardial cells of rats. METHODS: The cells were labelled with fluo-3-AM, and [Ca 2+ ]i was determined by use of confocal microscopy (ACAS 570). RESULTS: In the study, we found that [Ca 2+ ]i of myocytes was elevated 10min after adding PD (0.6 mmol/L), but [Ca 2+ ]i of some cells increased to 111.80%?2.22% vs baseline, the others to 224.00%?24.33%. The effect of PD was inhibited remarkably by pretreated with EGTA(2mmol/L), verapamil (50 ?mol/L), a kind of L-calcium channel antagnist, and tetrodotoxin ( 1 ?mol/L), a kind of sodium channel blocker 10 min before PD, the fluorescence value were decreased to 53.00%?9.02% , 52.00%?7.07% and 72.67%?12.70% respectively vs baseline (P
