Effects of aflatoxin G_1 on proliferation and TNF-? secretion of human peripheral blood mononuclear cells in vitro
- VernacularTitle:黄曲霉毒素G_1对体外培养HPBM增殖及TNF-?分泌的影响
- Author:
Huiyan WANG
;
Xianghong ZHANG
;
Xia YAN
;
Junling WANG
;
Xuming SUN
;
Yongbin YANG
;
Fengrong WANG
- Publication Type:Journal Article
- Keywords:
Monocytes;
Lymphocytes;
Aflatoxins;
DNA;
Tumor necrosis factor
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effects of aflatoxin G 1(AFG 1 )on proliferation and TNF-? secretion of human peripheral blood mononuclear cells(HPBM) in vitro. METHODS: The effects of AFG 1 on proliferation of HPBM were analysed with flow cytometric (FCM) DNA analysis and MTT bioassay, while that on TNF-? secretion was detected with ELISA. RESULTS: FCM analysis revealed that 6 h after treatment, proliferation index(PI) of 1000 ?g/L AFG 1 treated HPBM was significantly higher than that of control. 24 h after AFG 1 treatment, stimulating effects on proliferation was found in HPBM treated with AFG 1 at 200 ?g/L and 1 000 ?g/L.Regression analysis showed that PI was postively correlated with the concentrations of AFG 1 in the concentration range from 0 to 1 000 ?g/L( r=0. 5122 and 0.5119 respectively,P
