Allogeneic Mixed Melanocyte Lymphocyte Culture: An Experimental Study
- VernacularTitle:同种异体淋巴细胞与黑素细胞混合培养的实验研究
- Author:
Leihong XIANG
;
Zhizhong ZHENG
;
Luchuan ZHU
- Publication Type:Journal Article
- Keywords:
Melanocytes Lymphocytes Culture
- From:
Chinese Journal of Dermatology
1995;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the melanocyte lymphocyte reacion when the allogeneic lymphocytes are cultured with normal melanocytes. Methods 3H-thymidine was incorporated into the mixed culture and the transformation and proliferation rates of lymphocytes were detected by liquid scintillation counting and expressed as cpm. Electron microscopy was used to observe the ultrastructure of melanocytes after mixed culture. Results The results of lymphocyte proliferation were expressed by the stimulation indexes. The stimulation indexes in active vitiligo group was significantly different from that in stable vitiligo group and normal controls. The stimulation indexes of the melanocyte stimulated group was significantly different from that of ConA stimulated group. In the mixed melanocyte lymphocyte reaction, the allogeneic lymphocytes had little effect on the melanocytes. The ultrastructure of the melanocytes in the mixed culture showed normal morphology and normal function of synthesis of melanin. Conclusion As a specific antigen in mixed melanocyte lymphocyte reaction, melanocyte has a weak effect on the lymphocytes. The melanocytes from stable stage vitiligo patients seem more suitble to be allografted.
