Effects of propofol on brain dopamine transporter and serotonin transporter activity in mouse
- VernacularTitle:异丙酚对中枢多巴胺转运蛋白和5-羟色胺转运蛋白活性的影响
- Author:
Guoqin SUN
;
Huifang XU
;
Wenshan SUN
- Publication Type:Journal Article
- Keywords:
Propofol;
Brain;
Dopamine uptake inhibitor;
Serotonin uptake inhibitor
- From:
Chinese Journal of Anesthesiology
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the changes in brain dopamine transporter and serotonin transporter in mice receiving propofol anesthesia, 125I-?-CIT binding was studied.Methods Twenty-seven Kunming mice ,weighing 18-22g ,were randomly assigned to receive either intraperitoneal propofol 100 mg/kg (n=9) or 200 mg/kg(n=9) or 10% intralipid (used as control,n=9) in equal liquid volume respectively. 10 min after intraperitoneal administration, 0.1ml 2?Ci 125I-?-CIT was given intravenously via the tail vein. Two hours later animals were decapitated. Brains were rapidly removed and dissected on ice and cerebellum, hypothalamus, striatum and cerebral cortex were separated. Brain tissues were weighed and the radioactivity of 125I-?-CIT in different brain regions was measured.Results 125I-?-CIT binding to dopamine transporter or serotonin transporter decreased significantly in propofol groups as compared with that in control group. The decrease in binding was greater in 200 mg/kg propofol group (P
