Effects of human/mouse embryonic fibroblast cells on the growth of human embryonic stem cells
- VernacularTitle:人胚胎成纤维细胞对人胚胎干细胞生长的作用
- Author:
Ling XU
;
Shaoliang HUANG
;
Shunong LI
;
Xua WU
- Publication Type:Journal Article
- Keywords:
Embryo;
Stem cells;
Fibroblasts;
Human
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: In order to replace mouse embryonic fibroblast cells by human embryonic fibroblast cells to support the growth of human embryonic stem cells, the effects of human/mouse embryonic fibroblast cells on the growth of human embryonic stem cells were compared. METHODS: Both mouse and human embryonic fibroblast cells were used as feeder layer to support human embryonic stem cells. The proliferation and differentiation of human embryonic cells were observed. RESULTS: Combined use of human leukemia inhibitory factor with human/mouse feeder layer cells would support growth and proliferation of human embryonic stem cells and kept in undifferentiated condition. There was no difference between human/mouse cell. CONCLUSION: Human embryonic fibroblast cells can be used to support proliferation of human embryonic stem cells, eliminating the influence of foreign protein.
