Oxidant regulates macrophage colony-stimulating factor gene expression in RAW264.7 cells
- VernacularTitle:氧化剂对RAW264.7细胞M-CSF基因表达的调控
- Author:
Zhanjun PANG
;
Mei ZHOU
;
Yua CHEN
- Publication Type:Journal Article
- Keywords:
Macrophage colony-stimulating factor;
Oxidants;
Cell line
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To find out the effect of oxidative stress on macrophage colony-stimulating factor (M-CSF) gene expression in macrophages.METHODS: The effect of tert-butyl hydroperoxide (tbOOH) on M-CSF expression in RAW264.7 cells was investigated by RT-PCR method.RESULTS: The results showed that tbOOH (1 5?10 -4 mol/L) could induce M-CSF mRNA expression in RAW264.7 cells; and the induction became greater with increase in tbOOH concentration. Furthermore, the induction of M-CSF in RAW264.7 cells by tbOOH could be attenuated by cycloheximide or acetovanilone added in the medium.CONCLUSION: The results indicated that tbOOH could transcriptionally induce M-CSF expression in RAW264.7 cells, and de novo protein synthesis and intracellular superoxide (O 2) production might be involved in the process.
