The mechanism of intercellular adhesion molecule-1 expression in endothelial cells stimulated by advanced glycosylation end products
- VernacularTitle:糖基化终产物刺激大鼠骨髓内皮细胞表达细胞间粘附分子-1的机制探讨
- Author:
Lu YU
;
Hongxin QIU
;
Yaping WANG
;
Liangyi SI
;
Shan WU
;
Jihu ZHU
- Publication Type:Journal Article
- Keywords:
Glycosylation end products, advanced;
Cell adhesion molecule;
Endothelium;
Cells;
Free radicals
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the relationship between intercellular adhesion molecule-1(ICAM-1)expression in endothelial cells(EC) and advanced glycosylation end products(AGEs) stimulation. METHODS: Murine bone marrow derived ECs was stimulated by AGEs after pretreated with anti-AGEs, anti-IL-1? and N-acetylcysteine(NAC),then SOD activity and ICAM-1 concentration and adhesion rate(AR) were evaluated. RESULTS: ECs which expressed ICAM-1[(0.65?0.14) vs (0.11?0.02)] induced by AGEs showed lower SOD activity [(0.69?0.19)?10 3 U/L vs (1.71?0.42)?10 3 U/L]. The ICAM-1 expression as well as the increase of AR caused by AGEs stimulation could be suppressed by anti-AGEs(0.12?0.01) and NAC(0.11?0.05). Anti-IL-1? had no influence on these changes. CONCLUSION: AGEs could induce endothelial cells to express ICAM-1 in vitro, most probably due to the formation of free radicals. Besides, AGEs may stimulate other cells to secrete cytokines resulting in ICAM-1 expression in endothelial cells.
