Changes in L-arginine/NO pathway of erythrocytes in patients with essential hypertension
- VernacularTitle:原发性高血压患者红细胞L-精氨酸/一氧化氮系统的改变
- Author:
Jun WANG
;
Wenjie YU
;
Xinghai YAO
;
Ningling SUN
;
Chaoshu TANG
;
Yimi ZANG
- Publication Type:Journal Article
- Keywords:
Hypertension;
Erythrocytes;
Arginine;
Nitric oxide
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the changes of L-arginine/nitric oxide(L-Arg/NO) pathway and the characteristics of L-Arg transport in erythrocytes (RBC) in patients with essential hypertension(EH). METHODS: 12 EH patients were studied with 10 normotensive people as control. The nitric oxide (NO) production was assayed by measuring the nitrite in medium using Greiss reaction. NO synthase activity was measured according to the method described by Ghigo. In order to achieve zero-trans condition, erythrocytes were incubated at 37℃ for 3 h and then L- Arg transport of RBC were determined by measuring the influx of radiolabelled L-arginine by Deliconstantins described method.RESULTS: In EH patients, NO production was lower than that in control (P
