Role of renal cortex endothelin and NO in the development of proteinuria
- VernacularTitle:肾局部内皮素、一氧化氮在尿蛋白产生中的作用
- Author:
Jie ZHANG
;
Dan WANG
;
Henghao MA
;
Xiaochun WANG
- Publication Type:Journal Article
- Keywords:
Endothelins;
Nitric oxide;
Nephrotic syndrome;
Rats
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To test the hypothesis that locally produced renal endothelin(ET) and nitric oxide(NO) play more important role in initiating proteinuria than circulating ET and NO. METHODS: (1) Rat nephrotic syndrome model was made by single injection of adriamycin, and the urinary protein excretion per 24 hours for every two days was measured. (2) Rat plasma and renal cortex were collected respectively 4 days, 8 days, 32 days and 56 days after the injection, and renal cortex homogenate was further prepared; ET of both plasma and renal cortex homogenate were determined by radioimmunoassay; NO of both plasma and renal homogenate were measured by the method of Griess. (3) The correlation between urinary protein and ET or NO concentration was analyzed respectively. RESULTS: (1) The urinary protein excretion of normal rat was 4-7 mg/d, that in nephrotic rat at the 4 time points was 5, 10, 241 and 201 mg/d, there was a significant increase of that at day 8, peaked at day 32, and less decrease at day 56. (2) The plasma ET in nephrotic rats of the 4 time points was 134, 150, 538 and 445 ?g/L, respectively, which of day 8, day 32 and day 56 shown significant higher than that of normal control (126-129 ?g/L). (3) The ET in renal cortex homogenate of nephrotic rat of the 4 time points was 364 6, 652 3, 1 526 8, 1 393 6 pg/g, all of that was much more higher than that of normal rat [(235 9-246 1) pg/g,P
