Simultaneous Determination of Contents of Piperacillin and Tazobactam in a Preparation With RP-HPLC
- VernacularTitle:RP-HPLC法同时测定哌拉西林和他唑巴坦制剂中两组分的含量
- Author:
Zeming YANG
;
Jisheng CHEN
;
Shiming YU
;
Zhuoxia CHEN
- Publication Type:Journal Article
- Keywords:
piperacillin;
tazobactam;
RP_HPLC;
internal standard
- From:
China Pharmacy
1991;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a RP-HPLC method to simultaneously determine the contents of piperacillin and taz_obactam METHODS:Using ? Bondapak C18 column as fixed phase,a mixture of methanol and purified water(34 5∶65 5)as mobile phase,and caffeine as internal standard,detecting wavelength:220nm,flow rate:1 0ml/min RESULTS:The linear rangs of piperacillin and tazobactam were 40～200?g/ml and 5 0～25 0?g/ml respectively The average recovery and RSD (n=5) were (100 2?0 61)% for piperacillin and (99 03?0 90)% for tazobactam CONCLUSION:This method is rapid,simple and accurate
