A Study on Detection of Lymph Node Micrometastasis by Using Monoclonal Anti-keratin Antibody in Patients with Colorectal Cancer
- VernacularTitle:角蛋白单克隆抗体免疫组化法检测大肠癌淋巴结微转移的研究
- Author:
Bo LIU
;
Daojin CHEN
- Publication Type:Journal Article
- Keywords:
Colorectal;
Cancer,Keratin;
Node;
Micrometastasis;
Immunohistochemistry
- From:
Journal of Chinese Physician
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the significance of detection of lymph node micrometastasis by immunohistochemical method with monoclonal anti-karatin antibody in patients with colorectal cancer.Methods:We examined 331 lymph nodes obtained from 47 colorectal cancer patients who underwent a curative operation and diagosed by using immunohistochemistry SB techniques.Monoclonal mouse anti-keratin antibodies(AE1,AE1/AE3,AE3)were used as the primary reagents to identify tumor cells both in tumor tissues and lymph nodes.Results:A total of 36 micrometastasis among 331 lymph nodes(11 9%) were found.Duke's C staging patients had more lymph nodes metastasis than that in Duke's B staging patients(P
