Detection of Rpsl Gene Mutation of Mycobacterium Tuberculosis by PCR-SSCP Silver Staining
- VernacularTitle:PCR-SSCP银染法检测结核分枝杆菌rpsL基因突变
- Author:
Suling CHEN
;
Meirong ZHAO
;
Mingbao WANG
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
Resistance,Streptomycin;
Gene variati
- From:
Journal of Chinese Physician
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the value of detection of rpsl gene mutation in streptomycin(SM)-resistance in clinical isolates of mycobacterium tuberculosis by PCR-SSCP,and to expect to set up a rapid detective method of rpsl gene mutation in mycobacterium tuberculosis,Method Eighty seven specimens isolated from patients were detected by PCR-SSCP silver staining,and using strain H 37 Rv as a control,Results In 87 mycobacterium tuberculosis clinical isolates,the rpsl DNA fragments from 16 drug-susceptible isolates had no mutation in rpsL gene,48 of 62 sputum specimens showed rpsl gene mutation by PCR-SSCP,positive rate was 77 4%.Conclusions The rpsL gene mutation is an important molecular mechanism of M.tuberculosis resistance to SM.PCR-SSCP might become a rapid detecting method of SM-resistance of M.tuberculosis.
