Gene Amplification Test of Mycobacterium Leprae in Paraffin－ Embedded Tissue
- VernacularTitle:石蜡包埋组织中麻风菌基因扩增试验初探
- Author:
Qinxue WU
;
Yueping YING
;
Liangfen ZHANG
- Publication Type:Journal Article
- Keywords:
Mycobacterial leprae Polymerase chain reaction
- From:
Chinese Journal of Dermatology
2000;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a polymerase chain reaction(PCR) method to amplify DNA of M.leprae from fixed and paraffin－ embedded tissue(FPET). Methods The DNA of M.leprae was released from FPET by using Texpat Kit and purified with 100％ alcohol. The primers RPOT(1) and RPUT(2) were used to conduct the PCR. Results A total of 32 samples were examined. Out of 32 samples with BI of more than 1＋ , 28 were positive for PCR. The PCR was negative in a sample with BI=0. The sensitivity of PCR reached a level of 0.04 pg DNA. Conclusion This PCR method is very useful for amplifying the DNA of M.leprae from FPET.
