Detection of p53 and p21~(WAF1) in cholangiocarcinoma
- VernacularTitle:在胆管癌组织中检测p53,p21~(WAF1)的表达
- Author:
Xin WANG
;
Jianxi ZHANG
;
Jie CHEN
- Publication Type:Journal Article
- Keywords:
Gene p53;
Gene p21 WAF1;
Cholangiocarcinom?
- From:
Chinese Journal of Hepatobiliary Surgery
1998;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the clinical significance of p21 WAF1 and the relationship between it and p53. Methods Samples of human cholangiocarcinoma (HC) tissue and paired normal bile duct tissue adjacent to the tumor from 30 patients with HC were employed in this study. In situ hybridization (ISH) was used to detect the p53 and p21 mRNA expression. Immunohistochemistry (IHC) was applied to analyze p53 gene mutation and P21 protein expression. Results p21 WAF1 And p53 gene protein expression was detected in 36.7% (11/30) and 53.3% (16/30) of the carcinoma specimens, respectively, and none of the paired tissue by IHC. p53 Positivity was related to local lymph node metastasis. Comparing with non-lymph node metastatic group, p53 positivity in metastatic group was significantly higher. p53 Positivity of cholangiocarcinoma in clinical stage III was significantly higher than that in clinical stages I and II. The survival time was significantly shorter in patients with P53 protein expression than in those without. It was found that p53 expression was not associated with p21 WAF1 expression. Conclusions P53 Positivity may be correlated with tumor development but not tumor occurrence. The fact that p53 expression was not associated with p21 WAF1 expression indicates that p53-independent activation of p21 WAF1 may exist.
