Protective effects of protease inhibitor on liver function after ischemia reperfusion and orthotopic liver transplantation in rats
- VernacularTitle:蛋白酶抑制剂对大鼠肝缺血再灌注和原位肝移植后肝功能的保护作用
- Author:
Guichen LI
;
Yongfeng LIU
;
Jialin ZHANG
- Publication Type:Journal Article
- Keywords:
Reperfusion injury;
Liver transplantation;
Protease;
Aprotinin
- From:
Chinese Journal of Hepatobiliary Surgery
1998;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of neutrophils and protease inhibitor in the pathogenesis of hepatic ischemia reperfusion injury. Methods The models of liver ischemia reperfusion (LIR) and orthotopic liver transplantation (OLT) were established in rats. The contents of serum ALT and LDH and liver myeloperoxidase(MPO) were determined. Meanwhile, the hepatic pathohistological changes were observed and the number of infiltrating neutrophils counted. As for the LIR model, the animals were administered with aprotinin (AP) before ischemia. Then reperfusion was achieved 45 min after ischemia. The samples were collected after reperfusion for 4 h. In OLT model, the animals were administered with AP 1 h before the transplantation and the samples were collected 4 h postoperatively. Results The contents of serum ALT and LDH and liver MPO were significantly higher in the control group than in the experimental one (P
