Experimental induction of intestinal metaplasia in stomach＆ expression of tumor-related proteins
- VernacularTitle:犬胃粘膜肠上皮化生模型的建立及其肠化过程中癌相关蛋白的表达
- Author:
Dongxu WANG
;
Dianchun FANG
;
Weiwen LIU
- Publication Type:Journal Article
- Keywords:
Intestinal metaplasia;
Animal model;
Tumor-related gene;
Protein expression
- From:
Chinese Journal of Digestive Endoscopy
1996;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The development and progression of intestinal metaplasia ( IM ) and expression of tumor-related proteins in dog’ s stomach were observed. Methods IM animal model was established in Beagle’ s stomach by feeding N-methyl-N’-nitro-N-trosoguanidine( MNNG ),ranitidine,and local X-irradiation. Expression of APC, p53, K-ras and bcl-2 genes in canine gastric lesion were determined by immunohistochemistry. Resullts IM canine model was successfully induced, with which sequential pathology studied. It was proved that progression of normal epithelial cells to IM cells might undergo several stages including superficial gastritis, chronic atrophic gastritis, mild focal IM, moderate,and severe IM.Aberrant bcl-2 protein was detected in atrophic gastric epithelium and abnormal expression of APC, K-ras, and bcl-2 found in IM mucosa. Conclusion Canine gastric IM was induced by the method mentioned above.The development of normal gastric mucosa to IM in dog seemed to resemble that of human being.The presence of tumor-related proteins might indicate the transformation of IM to malignancy.
