A morphometric analysis of age-related changes in the RPE, Bruch's membrane and choroid of human eyes
- VernacularTitle:视网膜色素上皮、Bruch膜及脉络膜老年性改变的定量分析
- Author:
Yongjin ZHANG
;
Ireland GRENHAM
;
Boulton MIKE
- Publication Type:Journal Article
- Keywords:
Pigment epithelium of eye;
Choroid;
Bruch membrane;
Image processing, computer assisted
- From:
Chinese Journal of Ocular Fundus Diseases
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To identify and quantitatively evaluate age related changes in the retinal pigment epithelium (RPE) and underlying Bruchs membrane and choroid in donor human eyes. Methods 36unpaired human eyes of varying age (3～93 years) from Caucasian donors were supplied by Manchester Eye Bank (UK) or National Disease Research Interchange (Philadephia,USA).Modified Massons trichrome staining was used to illustrate age related changes in RPE cell, Bruchs membrane thickness, and density of choriocapillaries and thickness of the choroid. Data were assessed using computer aided quantitative morphometric analysis method. Results The thickness of Bruchs membrane increased with age while there is a change in morphology of RPE cells including a decrease in number and RPE cell thickening with age. RPE cells decreased at a rate of 8 cells/mm 2?year, RPE cell height and thickness of Bruchs membrane increased at rates of 0.01(?m/year) and 0.02 (?m/year) respectively. The luminal area of choriocapillaries and the thickness of choroid showed no close relation with age. Conclusion RPE cell loss and thickening of Bruchs membrane and RPE cells may be the earlier and primary alteration with age.
