A quantifying model of vascular proliferation in oxygen-induced retinopathy
- VernacularTitle:可定量的氧致血管增生性视网膜病变小鼠模型
- Author:
Xiaoyan PENG
;
Danian CHEN
;
Mi YAN
- Publication Type:Journal Article
- Keywords:
WT5”BZ]Retinal diseases;
Neovascularization;
Endothelium,vascular;
Angiogenesis factor;
Disease models,anim(
- From:
Chinese Journal of Ocular Fundus Diseases
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose [WT5”BZ]To estabalish a quantifying model of retinal neovascularization suitable for the study of pathogenesis and therapeutic intervention for the retinal neovascularization. [WT5”HZ]Methods [WT5”BZ]Sixteen one week old C 57 BL/6 mice were exposed to 75% oxygen for 5 days and then to room air and 16 mice of the same age kept in room air as controls.Ink perfused retinal flatmount was examined to assess the oxygen induced changes of retinal vessels.The proliferated neovascular response was quantitated by counting the nuclei of endothelial cells of new vessels extending from the retina into the vitreous in 6 ?m sagittal cross sections. VEGF and bFGF were determined on the cross sections after immunohistochemcal stain. [WT5”HZ]Results [WT5”BZ]Constriction and closure of the blood vessels were found under the hyperoxia condition,and dilation and proliferation were found under the relatively hypoxia status.There was a mean of 24 neovascular nuclei per cross section in the oxygen treated retina and less than 1 nucleus in the control group ([WT5”BX]P
