Analysis of mutation in short taudem repeat
- VernacularTitle:短串联重复基因座突变率的分析研究
- Author:
Jianjin LI
;
Xianglin WU
;
Dejian LU
- Publication Type:Journal Article
- Keywords:
Short tandem repeat;
Mutation;
Paternity test
- From:
Chinese Journal of Forensic Medicine
2000;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
By analysing the 300 cases of paternity test in our lab in the recent year, we have demonstrated that mutation rates in STR loci are very high. The experimental steps include extracting DNA by chelex 100, amplification of DNA, polyacrylamide gel electrophoresis, silver staining, DNA typing by standard samples. The STR mutations in 11 cases among 300 paternity test cases were found, induding D11S554 locus6 cases, D19S253 locus2 cases, SE33 locuslcase, D12S391 locus 1 case, D13S631 locus 1 case. The results indicated that high mutation rates in STR loci should be considered when STR genotyping are applied to paternity test.
