Investigation of species specificity on 20 PCR-based Human STR systems
- VernacularTitle:20个STR基因座的种属特异性研究
- Author:
Xianglin WU
;
Jianjin LI
;
Xinyao WU
- Publication Type:Journal Article
- Keywords:
STR (short tandem repeat);
Species specificity;
Forensic science
- From:
Chinese Journal of Forensic Medicine
2000;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
To evaluate species specificity of 20 STRs loci used in our lab. Human DNA and 10 different kinds of common animals' DNA were amplified separately. The PCR products were analysed with PAGE. There are amplified products in DYS388, DYS389, DYS390, D7S809, D13S631 loci of human 'DNA, but not of 10 different kinds of animal DNA tested. There were amplified products in TH01, vWA, AR, CD4, FABP loci for human and animals' DNA, but the lengths of amplified fragment were distinctly different between human and animals. There were products of FES, D12S391, CSF1PO, D19S253, D21S11, FGA, DYS19 loci for human and animals DNA, but the product lengths were not different between human and animals. There were PCR products in SE33 and D11S554 loci for human and animals, their amplified fragment lengths were within the same electrophoretic field. 10 STR loci, such as TH01 etc, were highly specific for human being and the amplified fragments lengths of 8 STR loci, such as FES etc, were different between human and animal tested.
