The practical use of fluorescence-based STR typing technique for decomposed tissues
- VernacularTitle:荧光标记STR分型技术检验腐败组织基因型
- Author:
Suibao WANG
;
Yue LI
;
Chao LIU
- Publication Type:Journal Article
- Keywords:
STR loci;
Fluorescein labelling;
Decomposed tissues;
Co-amplification
- From:
Chinese Journal of Forensic Medicine
2000;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
To detect STR genotypes of degraded DNA from decomposed tissues. The PCR apmlified products of 40 degraded DNA samples, which were purified from decomposed muscles after death for 1～6 weeks, were separated by denaturing polyacrylamide. The genotypes were analyzed by 377 DNA sequencer and 2 fluorescence-based multiplex amplification systems which contain 12 STR loci and lsex locus. The specific PCR products of 12 STR loci and 1 sex locus of the 40 samples were typed precisely and rapidly. The method described here is very useful in forensic case works, especially for the seriously degraded DNA samples.
