Effects of hyperbaric oxygen and cyclosporin A on the levels of active oxygens and nitric oxide in spleens of skin transplanted mice
- VernacularTitle:高压氧与环孢素A对皮肤移植小鼠脾活性氧及一氧化氮含量的影响
- Author:
Yanqiu YU
;
Wei TIAN
;
Haipeng ZHANG
;
Xuexin CHEN
- Publication Type:Journal Article
- Keywords:
Skin transplantation;
Nitric oxide;
Hyperbaric oxygenation;
Cyclosporine
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study effects of hyperbaric oxygen (HBO) and cyclosporin A (CsA) on the contents of active oxygens and nitric oxide (NO) in spleens of skin transplanted mice.METHODS: The donor mice BALB/C and receptor mice C 57BL/6 were tested for skin transplantation. The HBO group mice were treated with 99.2% oxygen under 0.25 MPa for 1.5 hours, while CsA group mice were treated with CsA 0.5 mg?kg -1?d by abdomen injection. After 14 days, the spleen were extracted the contents of malondialdehyde (MDA) and NO and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT) and NO synthases (NOS) were determined.RESULTS: (1) Compared with the control group, the transplantation group, HBO group and CsA group have markedly increased the content of MDA and the activities of GSH-PX and CAT; Compared with the transplantation group, the CsA group have markedly increased activity of SOD and reduced activities of GSH-PX and CAT; the HBO group have markedly reduced the activity of GSH-PX and increased the activities of CAT and SOD (P
