The clinical application of fluorescently-labeled monoclonal antibody against P-selectin
- VernacularTitle:抗P-选择素单抗的荧光素标记及其临床应用
- Author:
Yiming ZHAO
;
Mingqing ZHU
;
Yang HE
;
Wenhong SHENG
;
Changgeng RUAN
- Publication Type:Journal Article
- Keywords:
Antibodies,monoclonal;
Fluoresceins;
Flow cytometry;
Cell adhesion molecules
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the clinical significance in determination of the P-selectin levels in subjects with prethrombotic state or thrombosis by flow cytometry (FCM). METHODS: The P-selectin expression on platelet membrane in 42 patients with diabetes mellitus, 33 with hyperlipidemia, 23 with cerebral infarction and 20 healthy individuals, were analyzed using fluorescently-labeled SZ-51 by direct FCM comparing with indirect FCM and enzyme-linked immunosorbent assay (ELISA). RESULTS: The level of P-selectin on platelet membrane is higher in DM (23.92%?15.83%), in hyperlipidemia (18.34%?9.46%) and in cerebral infarction (19.32%?10.38%) than normal subjects (3.38%?1.11%) (P
