Effect of neuropeptide Y on bcl-2, bax, fas expressions and proliferation in vascular smooth muscl cells
- VernacularTitle:神经肽Y对bcl-2,bax,fas基因表达和血管平滑肌细胞增殖的作用
- Author:
Cong GAO
;
Minsheng CHEN
;
Shaohua HUANG
;
Jiankang LIU
- Publication Type:Journal Article
- Keywords:
Cells;
Apoptosis;
Neuropeptide Y;
Muscle, smooth, vascular
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the effect of neuropeptide Y on expression of apoptosis associated genes and proliferation in vascular smooth muscle cells(VSMC). METHODS: The proliferation activity of VSMC was dterminded by MTT colorimetry. The average fluorescence intensity that represented VSMC nuclear antigen (PCNA) and bcl-2, bax, fas expressions was quantitatively measured by fluorescence immunohistochemistry. RESULTS: Compared with control, the expressions of bcl-2, bax, fas, PCNA and the VSMC proliferation activity in VSMC treated with NPY were significantly increased. CONCLUSION:NPY may increase the expression of apoptosis associated genes in VMSC and promote its proliferation.
