Changes of acetylcholine receptor in skeletal muscle after upper motoneuron injury and the regulation of calcitonin gene related peptide
- VernacularTitle:上运动神经元损伤后骨骼肌AChR的改变及CGRP的调控作用
- Author:
Geng WANG
;
Xinmin WU
- Publication Type:Journal Article
- Keywords:
Calcitonin gene related preptide;
Receptors,cholinergic;
Neuromuscular junction
- From:
Chinese Journal of Anesthesiology
1997;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the time profile of the changes of mRNA of ? and ? subunit of acetylcholine receptor (AChR) in skeletal muscle and the level of calcitonin gene related peptide (CGRP) in spinal motoneuron after upper motoneuron injury. Method The spinal cord of adult male Wistar rats was transected on the lower thoracic segment. The spinal cords below the transection level and soleus muscles were sampled 0,1,2,4,7 and 14 days after surgery, respectively, to detect the levels of mRNA of ? and ? subunit of acetylcholine receptor in soleus muscle using Northern blot method,and CGRP level in spinal motoneuron using radioimmunoassay.Results The levels of mRNA of ? and ? subunit of acetylcholine receptor siginificantly increased after surgery, but the level of mRNA of ? subunit increased earlier and higher than ? subunit. The level of CGRP in spinal motoneuron markedly reduced after surgery, was maintained at 50 80% of normal level since the 2nd day after surgery. Conclusions The levels of AChR ? and ? subunit in skeletal muscle significantly increase after upper motoneuron injury, the expression of which CGRP may regulate.
